(Modified from a release published by The Nobel Prize)
October 8, 2014
The Royal Swedish Academy of Sciences has decided to award the Nobel Prize in Chemistry for 2014 to Eric Betzig; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA; Stefan W. Hell, Max Planck Institute for Biophysical Chemistry, Göttingen, and German Cancer Research Center, Heidelberg, Germany; and William E. Moerner, Stanford University, Stanford, CA, USA. Thet received the prize “for the development of super-resolved fluorescence microscopy.”
Earlier this year, Stefan Hell was a recipient of the 2014 Kavli Prize in Nanoscience, which he received with Thomas W. Ebbesen and Sir John B. Pendry “for their transformative contributions to the field of nano-optics that have broken long-held beliefs about the limitations of the resolution limits of optical microscopy and imaging."
Stefan Hell joins Thomas W. Ebbesen and Sir John B. Pendry in a discussion about stretching our understanding of what light can do and successfully challenging long-held beliefs about the limits of optical microscopy and imaging.
Surpassing the Limitations of the Light Microscope
For a long time optical microscopy was held back by a presumed limitation: that it would never obtain a better resolution than half the wavelength of light. Helped by fluorescent molecules the Nobel Laureates in Chemistry 2014 ingeniously circumvented this limitation. Their ground-breaking work has brought optical microscopy into the nanodimension.
In what has become known as nanoscopy, scientists visualize the pathways of individual molecules inside living cells. They can see how molecules create synapses between nerve cells in the brain; they can track proteins involved in Parkinson’s, Alzheimer’s and Huntington’s diseases as they aggregate; they follow individual proteins in fertilized eggs as these divide into embryos.
It was all but obvious that scientists should ever be able to study living cells in the tiniest molecular detail. In 1873, the microscopist Ernst Abbe stipulated a physical limit for the maximum resolution of traditional optical microscopy: it could never become better than 0.2 micrometres. Eric Betzig, Stefan W. Hell and William E. Moerner are awarded the Nobel Prize in Chemistry 2014 for having bypassed this limit. Due to their achievements the optical microscope can now peer into the nanoworld.
Two separate principles are rewarded. One enables the method stimulated emission depletion (STED) microscopy, developed by Stefan Hell in 2000. Two laser beams are utilized; one stimulates fluorescent molecules to glow, another cancels out all fluorescence except for that in a nanometre-sized volume. Scanning over the sample, nanometre for nanometre, yields an image with a resolution better than Abbe’s stipulated limit.
Eric Betzig and William Moerner, working separately, laid the foundation for the second method, single-molecule microscopy. The method relies upon the possibility to turn the fluorescence of individual molecules on and off. Scientists image the same area multiple times, letting just a few interspersed molecules glow each time. Superimposing these images yields a dense super-image resolved at the nanolevel. In 2006 Eric Betzig utilized this method for the first time.
Today, nanoscopy is used world-wide and new knowledge of greatest benefit to mankind is produced on a daily basis.
About the Recipients
Eric Betzig, U.S. citizen. Born 1960 in Ann Arbor, MI, USA. Ph.D. 1988 from Cornell University, Ithaca, NY, USA. Group Leader at Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Stefan W. Hell, German citizen. Born 1962 in Arad, Romania. Ph.D. 1990 from the University of Heidelberg, Germany. Director at the Max Planck Institute for Biophysical Chemistry, Göttingen, and Division head at the German Cancer Research Center, Heidelberg, Germany.
William E. Moerner, U.S. citizen. Born 1953 in Pleasanton, CA, USA. Ph.D. 1982 from Cornell University, Ithaca, NY, USA. Harry S. Mosher Professor in Chemistry and Professor, by courtesy, of Applied Physics at Stanford University, Stanford, CA, USA.