(Originally published by TU Delft)
January 9, 2015
Researchers of the Kavli Institute of Nanoscience of TU Delft have discovered that tetrasomes - proteins that play a crucial role in the folding of DNA - fluctuate spontaneously between a left-handed and a right-handed form. This discovery has changed our view of the organization of DNA in cells. The research, conducted in cooperation with the Innsbruck Medical University, has appeared this week in the journal Cell Reports.
The length of our DNA (about 1 meter) is enormous compared to the dimensions of a cell nucleus (several micrometers). To ensure that DNA fits in the cell, it is systematically wrapped around certain proteins, resulting in the formation of nucleosomes. However, this conglomeration of DNA by nucleosomes also decreases the accessibility of the DNA for reading out the genes, for example. A dynamic interplay between DNA and proteins leads to a good balance between compact DNA and accessible DNA, but this interplay is still not properly understood.
The Delft researchers can now study the dynamics of a single nucleosome on DNA. They succeeded in forming artificial nucleosomes on an individual piece of DNA while simultaneously measuring different properties of the DNA.
The method used is a recent variation of a magnetic tweezers technique, in which a single DNA molecule is attached to a glass plate at one end and to a magnetic ball at the other. The placement of a doughnut-shaped magnet directly above the magnetic ball not only extends the DNA, but also enables the magnetic ball to rotate freely, allowing spontaneous rotations of the DNA to be read out. By taking extremely accurate measurements of both the height and the rotation of the magnetic ball before, during and after the formation of nucleosomes and tetrasomes (intermediate forms of nucleosomes), the researchers succeeded in determining the stability of these structures.
While taking measurements, the researchers discovered that a nucleosome is formed in two steps. In the first step, a tetrasome is formed from four so-called histone proteins, while the second step involves the addition of four histones to create a complete nucleosome containing 50 nanometres of DNA wrapped around the protein disk in a leftward direction.
As expected, the nucleosome was very stable and even remained that way for hours. By only adding half of the proteins, however, it became possible to study the tetrasome intermediate form for the first time. This yielded a surprise - the rotation continued back and forth, even though the length of the DNA did not change any more. The rotation direction of the tetrasome kept spontaneously changing. A model of structural change within the tetrasome, the so-called 'waving arms model', has been created to explain this movement.
The fact that reading out the genes in the DNA causes a great deal of twist in the DNA is a problem in cell biology that has remained unsolved for a long time. This applied twist causes problems for the functioning of the motor proteins that need to do their work on the DNA. Nevertheless, this does not seem to cause any difficulties for living cells. The fact that tetrasomes are able to 'absorb' this twist now seems to offer a good explanation for this robust functioning of our DNA.
This study was led by doctoral candidate Rifka Vlijm and post-doc Mina Lee and involved collaboration between the TU Delft groups of prof. Cees Dekker, who initiated the nucleosome research, and prof. Nynke Dekker, who developed the special magnetic tweezers measurement technique. The proteins were purified by prof. Alexandra Lusser of Innsbruck Medical University.